TUBB anticorps (N-Term)

N° du produit ABIN110979

Cet anticorps anti-TUBB est un anticorps Souris Monoclonal détectant TUBB dans WB, IF et IHC (p). Adapté pour Porc.

Aperçu rapide pour TUBB anticorps (N-Term) (ABIN110979)

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TU-06

Détail du produit anti-TUBB anticorps

Épitope: N-Term

Specificité: The antibody recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.

Purification: Precipitation methods

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Beta-subunits of porcine brain tubulin

Isotype: IgM

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): 5 μg/mL, Positive tissue: heartImmunocytochemistry: 2 μg/mL. Staining technique: fixed and permeabilized cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: TUBB / TUBB5

Sujet: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain

ID gène: 203068

NCBI Accession: NP_821133

UniProt: P07437

Pathways: Dynamique des Microtubules, M Phase